Counting sgRNA abundance

Tools Needed

For this step of the analysis we'll need two different tools:

1. fxtools
2. sgcount

Each of these tools has their own documentation (fxtools and sgcount) for more detailed usage - but I will describe everything you need for your analysis here.

Installation

If you've never used any rust tools before - you will need to install cargo the rust package manager.

# installs `cargo`
curl --proto '=https' --tlsv1.2 -sSf https://sh.rustup.rs | sh

Then we can install these tools easily with cargo

cargo install fxtools
cargo install sgcount

You can validate that they installed with the following:

fxtools --version
sgcount --version